Allele-Specific rpoB PCR Assays for Detection of Rifampin-Resistant Mycobacterium tuberculosis in Sputum Smears
نویسندگان
چکیده
منابع مشابه
Allele-specific rpoB PCR assays for detection of rifampin-resistant Mycobacterium tuberculosis in sputum smears.
We describe an allele-specific PCR assay to detect mutations in three codons of the rpoB gene (516, 526, and 531) in Mycobacterium tuberculosis strains; mutations in these codons are reported to account for majority of M. tuberculosis clinical isolates resistant to rifampin (RIF), a marker of multidrug-resistant tuberculosis (MDR-TB). Three different allele-specific PCRs are carried out either ...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2003
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.47.7.2231-2235.2003